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[(from)-253.2(PBMCs)-250(by)-249.9(adherence)-257.6(to)-250(plastic)-252.1(culture)-252.2(asks)-253.8(and)-246.4(were)]TJ
0 -1.1116 TD
[(differentiated)-325.8(into)-321.4(immature)-324.9(DCs)-321.8(by)-319(culturing)-322.5(for)-320(67)-320.1(days)]TJ
T*
[(in)-238.5(the)-240.7(presence)-243.6(of)-232.6(IL-4)-241.7(and)-240.6(granulocyte-macrophage)-246.9(colony-)]TJ
0 -1.1174 TD
[(stimulating)-504.5(factor)-504.3(\(GM-CSF;)-506.7(300)-498.6(and)-499.8(450)-170.3(U/mL,)-500.8(re)19.7(sp)15.3(ec)18.5(-)]TJ
0 -1.1116 TD
[(ti)20.6(ve)19.7(ly)15(;)-243.1(S)0(c)19.8(h)0(e)19.7(r)0(i)20.7(n)0(g)20.8(-)0(P)15.3(l)0(o)20.7(u)0(g)20.8(h)-243(In)20.9(te)19.6(rn)15.1(at)19.5(io)20.7(na)19.7(l\))-232.7(in)-232.7(RP)20(MI)-233.5(16)15.1(40)-232.6(me)18.4(di)20.7(um)]TJ
T*
[(co)19.7(nt)20.7(ai)13.8(ni)20.7(ng)-526.3(10)20.8(%)-537.8(F)0(C)14.2(S)0(.)-528.5(N)0(o)20(n)0(a)19.7(d)0(h)20.8(e)0(r)19.7(e)0(n)19.7(t)-542.6(ce)18.5(ll)20.6(s)-536.6(p)0(e)19.7(r)0(i)15(p)0(h)20.8(e)0(r)19.7(a)0(l)-527.6(b)0(l)15(o)0(o)20.8(d)]TJ
T*
[(ly)20.7(mp)19.6(ho)20.8(cy)13.9(te)19.6(s)-323.4(\()0(P)21(B)0(L)18.6(s)0(\))-313(w)0(e)18.8(r)0(e)-314.4(c)0(o)19.7(l)0(l)14.8(e)0(c)18.5(t)0(e)19.6(d)-323.6(fo)20.9(r)-323.6(l)0(a)19.6(t)0(e)19.6(r)-323.6(us)15.3(e.)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 62.022 194.9669 Tm
[(Mixe)-8.2(d)-330(lym)-8.2(phocyte)-340.7(reaction)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 177.1653 Tm
[(Monocyte-depleted)-486.6(PBLs,)-476.3(isolated)-483.6(from)-477.9(buffy)-483.5(coats)-480.1(of)]TJ
-1.0137 -1.1174 TD
[(healthy)-419.2(donors,)-418.8(were)-422.3(used)-420.1(in)-417(mixed)-422.7(lymphocyte)-418(reactions)]TJ
0 -1.1116 TD
[(\()-81.7(MLRs\).)-345.9(PBLs)-340.3(\(10)]TJ
7.3823 0 0 6.5614 138.6141 159.5905 Tm
(5)Tj
9.843 0 0 9.843 142.3559 155.2252 Tm
[(\))-335.1(were)-341.6(cocultured)-343(with)-339.6(6700)-338.5(allogeneic)]TJ
-8.1615 -1.1116 TD
[(DCs)-373.6(\(ratio)-370.8(of)-370.8(15:1\))-374.2(for)-371.9(46)-371.9(days)-368.2(and)-373.1(proliferation)-374.2(was)-373.8(as-)]TJ
0 -1.1174 TD
[(sessed)-647.7(by)-635.8([)]TJ
7.3823 0 0 6.5614 112.8755 137.707 Tm
(3)Tj
9.843 0 0 9.843 116.6173 133.285 Tm
[(H]-thymidine)-644.6(\(0.037)-164.4(MBq)-644.6([1)]TJ
/F7 1 Tf
12.5389 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(Ci/well];)-647.2(MP)]TJ
-18.6384 -1.1116 TD
[(Biomedicals\))-364.7(incorporation)-368.3(for)-360.3(16)-169.2(h.)-355.9(In)-365(USSC/MLR)-365(cocul-)]TJ
T*
[(ture)-322.4(experiments,)-319.8(MLRs)-318.4(were)-324.4(performed)-318.6(in)-319.1(the)-315.6(presence)-324.3(of)]TJ
T*
[(diminishing)-313.2(concentrations)-314(of)-307.4(USSCs)-306.4(\(6700,)-308.4(2220,)-307.3(and)-309.7(740)]TJ
0 -1.1174 TD
[(per)-303.9(well\).)-310.7(As)-302.4(a)-307.5(positive)-303.9(control,)-306.3(PBLs)-305.8(were)-307.1(stimulated)-308.6(with)]TJ
0 -1.1116 TD
(5)Tj
/F7 1 Tf
.6681 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(g/mL)-358.3(of)-359.3(PHA.)-353.9(All)-361.5(cultures)-362.5(were)-358.9(performed)-359(\(at)-361.7(least\))-360.3(in)]TJ
-1.2211 -1.1116 TD
[(quadruplicate)-405.2(in)-405.5(normal)-400.8(USSC)-403.4(medium)-402(\(ie,)-405.4(without)-406.4(dexa-)]TJ
0 -1.1174 TD
[(methasone\).)-243.7(Where)-246.2(indicated)-244.2(a)-244.2(neutralizing)-241.8(antibody)-246.4(against)]TJ
25.504 67.9242 TD
(TGF-)Tj
/F7 1 Tf
2.2232 0 TD
(b)Tj
/F4 1 Tf
.8236 0 TD
[(\(AB-101-NA,)-283(R&D)-277.1(systems;)-275.8(5)]TJ
/F7 1 Tf
12.6829 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(g/mL\))-273(was)-281.6(added)-272.8(at)]TJ
-16.2827 -1.1174 TD
[(cocul)-8(ture)-501(onset.)-505.4(PGB2,)-504.9(and)-505.6(the)-505.7(thromboxane)-505.4(antagonists)]TJ
0 -1.1116 TD
[(L655,240)-6.6(,)-331.7(ICI)-338.3(192,)-335(L670,596,)-338.3(and)-338.5(AA)-332.2(2414)-338.5(were)-335.9(obtained)]TJ
T*
[(from)-339.6(Cayman)-333.7(Chemical)-337.4(Company.)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 313.0582 669.8833 Tm
[(Tra)-9(nswell)-337.6(analysi)-9.3(s)-336.5(o)0(f)-333.6(USSC)-9(-associa)-8.9(ted)]TJ
0 -1.2007 TD
[(s)-6.5(uppressi)-8.1(ve)-330.8(ac)-6.4(tivity)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 640.1195 Tm
[(In)-376.5(Transwell)-383(experiments,)-377.4(respon)-7.5(der)-373.1(PBLs)-380.7(were)-376.2(labeled)]TJ
-1.0137 -1.1116 TD
[(with)-541.2(CFSE)-536.2(before)-544.5(use)-534.2(in)-538(MLR.)-539.8(To)-539(this)-534.4(purpose,)-542(PBLs)]TJ
0 -1.1174 TD
(\(5)Tj
/F6 1 Tf
1.0022 0 TD
()Tj
/F4 1 Tf
.72 0 TD
(10)Tj
7.3823 0 0 6.5614 339.8739 622.5447 Tm
(6)Tj
9.843 0 0 9.843 346.7905 618.1794 Tm
[(cells/mL\))-337.4(were)-335.9(resuspended)-337.7(in)-330.6(PBS)-332.3(containing)-330.5(1%)]TJ
-3.427 -1.1116 TD
[(of)-272.9(FCS.)-272.4(CFSE)-277(\()-81.7(Molecular)-279.5(Probes,)-270(Inc.\))-273.9(was)-275.9(added)-272.8(to)-273(a)-273(nal)]TJ
T*
[(concentration)-364.9(o)16.2(f)-340.9(5)]TJ
/F7 1 Tf
7.6431 0 TD
(m)Tj
/F4 1 Tf
.5357 0 TD
-.0158 Tc
[(M,)-359.1(and)-356.7(t).3(he)-363.6(cells)-356.5(w)-15.8(e)8.8(re)-357.9(gen)-15.8(t)10.7(ly)-356.7(mix)-15.8(e)9.6(d)-356.7(a)-.8(nd)]TJ
-8.1788 -1.1174 TD
-.0159 Tc
[(i)-15.9(n)10.6(cuba)-15.9(t)9.4(e)-.9(d)-431.7(for)-431.7(10)-166.7(mi)-15.9(n)-421.4(a)-.9(t)-431.8(3).3(7)]TJ
/F6 1 Tf
10.9377 0 TD
0 Tc
()Tj
/F4 1 Tf
.3801 0 TD
.4166 Tc
[(Ci)432.6(naC)431.8(O)]TJ
7.3823 0 0 6.5614 468.5668 583.6534 Tm
0 Tc
(2)Tj
9.843 0 0 9.843 476.3337 585.2975 Tm
-.0158 Tc
[(i)-15.8(n)10.7(cubat)-15.8(o)10.7(r)-431.6(prot)-15.8(e)9.5(c)-.8(ted)]TJ
-16.588 -1.1116 TD
-.016 Tc
[(f)-16(r)10.7(om)-294.8(ligh)-16(t)10.5(.)-290.1(A)-.7(d)-16(d)10.6(ing)-293.6(e)-1(qu)-16(a)9.4(l)-293.7(amount)-16(s)-277.3(o)-16(f)-283.1(F).3(CS)-293.4(stop)-16(p)10.6(e)-1(d)-293.6(l).1(abe)-16(l)9.3(ing)-293.6(o).2(f)]TJ
T*
[(c)-16(e)8.3(lls,)-393.8(and)-391.5(t).1(he)-392.6(cell)-16(s)-375.2(w)-16(e)8.6(re)-392.6(subs)-16(e)9.6(quent)-16(l)10.3(y)-391.5(washe)-16(d)-376.5(tw)-16(i)9.6(c)-1(e)-392.6(a)-1(nd)-391.5(re-)]TJ
0 -1.1174 TD
-.0156 Tc
[(s)-15.6(u)11.2(spe)-15.6(n)9.8(ded)-270.1(i).5(n)-270.1(n)-15.6(o)11(r).6(mal)-270.3(U)-15.6(S)10.3(SC)-271.2(medi)-15.6(u)10.9(m.)-272.5(CFSE-l)-15.6(a)9.7(b).6(eled)-275.9(respo)-15.6(n)11(der)]TJ
0 -1.1116 TD
[(P)-15.6(B)10.1(Ls)-275.7(were)-277(then)-275.9(cocu)-15.6(l)10.9(t).4(ure)-15.6(d)-260.9(w)-.3(i)-15.6(t)10.7(h)-270.1(D)-.3(C)-15.6(s)-260.5(i)-15.6(n)-259.8(th)-15.6(e)-260.9(l).5(ow)-15.6(e)9(r)-270.1(c)-15.6(h)9.8(amber)-275.9(of)]TJ
T*
-.0159 Tc
[(a)-254.3(24-we)-15.9(l)9.4(l)-247.5(p).3(la)-15.9(t)9.4(e)-248.5(cont)-15.9(a)9.4(i).2(nin)-15.9(g)-237(a)-248.5(T)-.9(ran)-15.9(s)10.9(well)-253.3(inse)-15.9(r)9.6(t)-247.5(\(Co)-15.9(s)10.9(tar,)-249.7(Co)-15.9(r)10.7(n).3(ing)-15.9(,)]TJ
T*
-.0157 Tc
[(I)-15.7(n)10.9(c.\).)-341.7(In)-345.1(the)-346.3(upp)-15.7(e)9.7(r)-339.3(ch)-15.7(a)9.7(m)-.7(ber,)-347.4(USSCs)-344.9(w)-.4(er)-15.7(e)-330.1(a)-.7(dd)-15.7(e)9.7(d)-339.3(at)-345.2(a)-346.3(r).5(atio)-345.1(of)]TJ
0 -1.1174 TD
[(1)-15.7(5)10.9(PBLs)-15.7(:)11(1).5(DC:1)-15.7(U)10(SSC.)-451.1(T)-.7(he)-455.7(USSCs)-454.3(w)-.4(ere)-449.9(s)-15.7(e)9.9(p).5(ara)-15.7(t)9.6(ed)-448.8(fr)-15.7(o)10.9(m)-450(the)]TJ
0 -1.1116 TD
[(o)-15.8(t)10.7(her)-425.8(t)-15.8(w)9.8(o)-425.8(cel)-15.8(l)-415.7(t).3(ypes)-431.4(b).4(y)-425.8(a)-427(s)-15.8(e)9.8(miperm)-15.8(e)8.4(a)-.8(ble)-427(m)-.8(e)-15.8(m)8.4(brane)-427(w)-15.8(i)9.8(th)-425.8(a)]TJ
T*
0 Tc
[(po)26.6(r)16.2(e)-290.2(s)16.4(i)16.1(ze)-275.2(of)-278.6(0)16.2(.)13.9(4)]TJ
/F7 1 Tf
6.3126 0 TD
(m)Tj
/F4 1 Tf
.5357 0 TD
-.0157 Tc
[(m.)-301.4(A)-15.7(f)10.1(ter)-304.7(7)-304.8(day)-15.7(s)-294.2(of)-304.8(cult)-15.7(u)10.8(r).5(e,)-307.1(CFSE)-306(cont)-15.7(e)9.6(n).5(t)-304.9(o).5(f)]TJ
-6.8483 -1.1174 TD
0 Tc
[(ce)24.3(l)16(l)16.1(s)-317.7(w)15.3(as)-308.4(d)16.2(e)15(t)16.1(e)15(rm)25.4(i)16.1(n)16.2(e)15(d)-317.9(u)16.2(s)0(i)26.7(n)16.2(g)-317.9()16.3(ow)-302.6(cy)25.4(t)16.1(o)16.2(m)15(e)15(t)0(r)26.5(y)16.2(.)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 313.0582 452.8062 Tm
[(Cond)-10.3(itioned)-341.3(medium)-340.9(analysis)-340.1(of)-333.6(USS)-10(C-associ)-7.9(ated)]TJ
0 -1.2007 TD
[(s)-6.5(uppressi)-8.1(ve)-330.8(ac)-6.4(tivity)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 423.0424 Tm
[(To)-429.6(produce)-430.5(co)19.7(nd)20.8(it)20.6(io)15(ne)19.7(d)-415.8(m)0(e)18.4(d)0(i)20.7(a)0(,)-408.9(U)0(S)20.1(S)0(C)20(s)-421.4(we)18.8(re)-406.5(cu)19.7(lt)14.8(ur)20.9(ed)-406.5(at)]TJ
-1.0137 -1.1174 TD
[(70)15.1(%)19.8(90)20.8(%)-520.5(c)0(o)19.7(n)0()21(u)0(e)19.7(n)0(c)19.7(e)-520.6(fo)20.9(r)-519.4(4)-519.5(da)19.7(ys)21(.)-521.8(T)0(h)19.6(e)-520.6(co)19.7(nd)20.8(it)20.6(io)20.7(ne)19.7(d)-519.5(m)0(e)18.4(d)0(i)20.7(a)]TJ
0 -1.1116 TD
[(fr)15.1(om)-400.8(th)20.7(e)-411.2(c)0(u)13.9(l)0(t)20.6(u)0(r)20.9(e)0(s)-400.6(w)0(e)13(r)0(e)-400.7(t)0(h)20.7(e)0(n)-406.5(r)0(e)19.7(c)0(o)19.7(v)0(e)19.7(r)0(e)19.7(d)-410(an)19.7(d)-415.8()0(l)20.9(t)0(e)19.5(r)0(e)19.7(d)-415.8(th)20.7(ro)20.9(ug)20.8(h)]TJ
T*
[(0.)18.5(45)20.8(-)]TJ
/F7 1 Tf
2.0332 0 TD
(m)Tj
/F4 1 Tf
.5472 0 TD
[(m)-330.6()0(l)15.1(t)0(e)19.5(r)0(s)-324.5(a)0(n)19.7(d)-335.2(us)21(ed)-331.7(im)19.5(m)15(e)0(d)13.9(i)0(a)19.6(t)0(e)19.5(l)0(y)20.7(.)-337.5(In)-324.7(so)21(me)-327.1(ex)19.7(pe)19.7(ri)20.7(me)18.4(nt)20.7(s,)]TJ
-2.5804 -1.1116 TD
[(co)13.9(nd)20.8(it)20.6(io)20.7(ne)19.7(d)-381.2(m)0(e)18.4(d)0(i)20.7(u)0(m)-372(w)0(a)18.8(s)-381(us)21(ed)-372(th)20.7(at)-372.1(wa)18.8(s)-381(h)0(e)19.7(a)0(t)-372.1(a)0(c)18.5(t)0(i)20.6(v)0(a)19.7(t)0(e)19.5(d)-381.2(by)-370.8(a)]TJ
0 -1.1174 TD
[(10)15.1(-m)19.6(in)-376.7(in)20.7(cu)19.7(ba)19.7(ti)20.6(on)-382.3(at)-377.9(85)]TJ
/F6 1 Tf
9.8606 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
[(C)-388([)0(2)20.9(8)0(])20.9(.)-389.3(Re)18.6(sp)21(on)20.8(de)19.7(r)-392.7(P)0(B)20(L)0(s)-377.6(w)0(e)18.8(r)0(e)-377.7(c)0(o)19.7(-)]TJ
-10.2465 -1.1116 TD
[(cu)13.9(lt)20.6(ur)20.9(ed)-256.8(wi)19.8(th)-255.8(DC)18.9(s)-265.9(i)0(n)-250(t)0(h)15(e)-261.4(US)20.1(SC)14.2(-c)19.7(on)20.8(di)20.7(ti)20.6(on)20.8(ed)-256.8(me)18.4(di)20.7(a.)-259.1(On)-250.7(da)19.7(y)-266(7)]TJ
T*
[(of)-376.5(cu)19.7(lt)20.6(ur)20.9(e)-382.4(i)0(n)-370.9(t)0(h)20.7(e)-382.4(co)19.7(nd)15.1(it)20.6(io)20.7(ne)19.7(d)-381.2(m)0(e)18.4(d)0(i)20.7(a)0(,)-374.3(p)0(r)20.9(o)0(l)20.7(i)0(f)20.7(e)0(r)19.7(a)0(t)13.8(i)0(o)20.7(n)-381.2(of)-370.8(th)20.7(e)-382.4(r)0(e)19.7(-)]TJ
0 -1.1174 TD
[(sp)15.3(on)20.8(de)19.7(r)-294.8(P)0(B)14.2(L)0(s)-285.4(w)0(a)18.8(s)-300.4(me)18.5(as)19.9(ur)20.9(ed)-291.3(by)]TJ
7.3823 0 0 6.5614 441.8077 339.7039 Tm
(3)Tj
9.843 0 0 9.843 445.4361 335.3385 Tm
[(H-)20(th)20.7(ym)19.6(id)20.7(in)20.7(e)-301.8(i)0(n)20.7(c)0(o)19.7(r)0(p)20.9(o)0(r)20.9(a)0(t)19.5(i)0(o)15(n)0(.)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 313.0582 312.4346 Tm
[(Cyt)-9(ometric)-338.8(bead)-335.7(array)-341.9(measur)-6.9(ement)-7(s)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 294.633 Tm
[(Th1)-350.1(\(IL-2,)-349.9(IFN-)]TJ
/F7 1 Tf
6.6179 0 TD
(g)Tj
/F4 1 Tf
.4953 0 TD
[(,)-349(and)-350(TNF-)]TJ
/F7 1 Tf
4.6135 0 TD
(b)Tj
/F4 1 Tf
.5529 0 TD
[(\))-352.4(and)-350(Th2)-344.3(cytokines)-352.1(\(IL-4,)]TJ
-13.2934 -1.1174 TD
[(IL-5,)-510.1(and)-511.3(IL-10\))-508.8(were)-508.7(quantied)-507.7(simultaneously)-514.5(using)-506.5(a)]TJ
0 -1.1116 TD
[(human)-520.5(Th1/Th2)-517.1(cytokine)-512.5(Cytometric)-522.8(bead)-513.6(array)-520.4(kit)-516.2(and)]TJ
T*
[(cytometric)-240.7(bead)-237.1(array)-238.2(software)-242.3(\(BD)-235.6(Biosciences\).)-241.1(This)-235.9(array)]TJ
0 -1.1174 TD
[(kit)-239.7(provides)-240.3(a)-238.4(mixture)-240.7(of)-238.3(six)-239.4(microbeads)-9.9(.)-233.8(Beads)-244.8(with)-235.9(distinct)]TJ
0 -1.1116 TD
[(uorescent)-429(in)20.7(te)19.6(ns)15.3(it)20.6(ie)19.5(s)-415.6(\()0(F)21(L)0(-)19.7(3)0(\))-399.6(a)0(r)14(e)-411.2(pr)20.9(ec)18.5(oa)19.7(te)19.5(d)-415.8(w)0(i)19.8(t)0(h)-399.8(a)0(n)13.9(t)0(i)20.6(b)0(o)20.8(d)0(i)20.7(e)0(s)]TJ
T*
[(sp)15.3(ec)18.5(i)20.9(c)-296(f)0(o)20.9(r)-300.6(ea)18.5(ch)-285.6(cy)19.7(to)20.7(ki)20.7(ne)19.7(.)-297.2(F)0(i)20.9(f)0(t)15(y)-294.8(mi)19.5(cr)19.7(ol)20.7(it)20.6(er)19.7(s)-300.4(o)0(f)-284.4(s)0(u)21(p)0(e)19.7(r)0(n)15.1(a)0(t)19.5(a)0(n)19.7(t)-295(of)]TJ
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[(cr)14(oa)19.7(rr)20.9(ay)-573.6(da)19.7(ta)-573.7(we)18.8(re)-573.5(de)19.7(po)20.8(si)20.9(te)13.8(d)-577.1(t)0(o)-572.5(G)0(E)18.8(O)-583.7(at)-573.7(th)20.7(e)-584(N)0(C)18.9(B)0(I)-573.4(s)0(i)20.9(t)0(e)]TJ
T*
[(\(G)14.2(SE)19.8(23)20.8(36)20.8(6\))20.9(.)-291.4(U)0(R)18.9(L)0(:)-280(w)0(w)19.1(w)0(.)17.6(n)0(c)19.7(b)0(i)20.7(.)0(n)18.5(l)0(m)19.5(.)0(n)18.5(i)0(h)20.7(.)0(g)18.5(o)0(v)20.8(/)0(g)20.7(e)0(o)19.7(/)0(q)15(u)0(e)19.7(r)0(y)20.9(/)0(a)19.6(c)0(c)18.5(.)0(c)17.3(g)0(i)]TJ
0 -1.1174 TD
[(?a)18.5(cc)]TJ
/F6 1 Tf
1.8201 0 TD
(=)Tj
/F4 1 Tf
.6048 0 TD
[(GS)14.4(E2)19.6(33)20.8(66)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 59.7543 366.8597 Tm
[(Size)-340.2(determ)-9.7(ination)-341.3(of)-333.6(the)-333.5(fact)-7.8(or)]TJ
/F4 1 Tf
9.843 0 0 9.843 69.7323 349.0582 Tm
[(Supernatant)-269.1(of)-261.4(DC-T)-260.9(cellUSSC)-267.5(cultures)-264.6(was)-264.3(centrifuged)]TJ
-1.0137 -1.1116 TD
[(over)-374.2(lters)-370.5(with)-368.4(a)-370.9(dened)-365.9(pore)-374.2(size)-369.5(\(Vivaproducts\),)-373.4(which)]TJ
0 -1.1174 TD
[(yields)-411(a)-405.4(ow)-407.2(through)-405.2(of)-411.1(a)-405.4(given)-404.2(molecular)-412.3(weight)-406.3(cutoff.)]TJ
0 -1.1116 TD
[(The)-339.7(ow)-332.3(through)-330.4(was)-339.2(tested)-337.3(in)-330.6(an)-337.4(MLR.)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 59.7543 293.2723 Tm
[(Lipi)-7.8(d)-335.7(analysis)-340.1(of)-333.6(cultu)-8.2(re)-332.8(sup)-6.4(ernatants)]TJ
/F4 1 Tf
9.843 0 0 9.843 69.7323 275.4141 Tm
[(Supernatant)-401.6(of)-393.8(DCs)-396.7(cultured)-397.3(together)-403(with)-391.4(allogeneic)-402(T)]TJ
-1.0137 -1.1116 TD
[(cells,)-293.6(and)-286.7(DCs)-287.2(cultured)-287.8(together)-287.8(with)-287.8(allogeneic)-286.8(T)-290.3(cells)-284.4(and)]TJ
T*
[(USSCs)-404.3(were)-405(collected)-406.6(after)-405.3(6)-398.5(days.)-400.5(After)-405(a)-399.7(centrifugation)]TJ
0 -1.1174 TD
[(step)-437.5(to)-440.1(remove)-436.4(cells)-439.9(and)-436.4(debris,)-437.3(the)-442.3(supernatant)-436.1(was)-442.9(col-)]TJ
0 -1.1116 TD
[(lected)-396.3(and)-396.1(frozen.)-392.4(Frozen)-394.6(samples)-396.9(were)-393.5(packed)-396(in)-394(dry)-389.2(ice)]TJ
T*
[(and)-321.2(shipped)-319.8(to)-319.1(Stockholm.)-315.3(Analysis)-321.7(of)-319(86)-319(lipid)-316.9(metabolites)]TJ
0 -1.1174 TD
[(wa)18.8(s)-335(a)0(c)18.5(c)0(o)19.7(r)0(d)20.9(i)0(n)15(g)-329.4(to)-319.1(Lu)19.6(nd)20.8(st)15.1(ro)]TJ
10.3099 .023 TD
()Tj
.4032 -.023 TD
[(m)-330.6(e)0(t)-326(a)0(l)19.5(.)-331.7([2)20.9(9])20.9(.)-331.7(C)0(o)19.8(n)0(t)20.7(r)0(o)20.9(l)0(s)-324.7(f)0(o)20.9(r)-335.1(ex)19.7(tr)20.7(ac)18.5(-)]TJ
-10.7131 -1.1116 TD
[(ti)14.8(on)-313.2(an)19.7(d)-323.6(y)0(i)20.7(e)0(l)19.5(d)-323.6(we)18.8(re)-314.3(ru)20.9(n)-323.6(i)0(n)-313.3(p)0(a)19.7(r)0(a)19.7(l)0(l)14.8(e)0(l)19.5(.)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 59.7543 175.8047 Tm
[(Stati)-10.7(stical)-337.2(analysi)-9.3(s)]TJ
/F4 1 Tf
9.843 0 0 9.843 69.7323 157.9464 Tm
[(A)-324.5(one-way)-327.7(ANOVA)-332.4(test)-328.2(was)-321.9(used)-327.9(to)-324.9(test)-328.2(the)-327.1(probability)]TJ
-1.0137 -1.1116 TD
[(of)-422.6(signicant)-428.1(differences)-425.5(between)-426.9(samples.)-423.4(Statistical)-425(sig-)]TJ
T*
[(nicance)-451.2(\()]TJ
/F9 1 Tf
4.4062 0 TD
(P)Tj
/F6 1 Tf
.7776 0 TD
(<)Tj
/F4 1 Tf
.72 0 TD
[(0.05\))-445.5(is)-445.6(indicated)-445.8(in)-445.8(the)-442.3(gures)-445.1(as)-446.7(follows:)]TJ
-5.9037 -1.1174 TD
(***)Tj
/F9 1 Tf
1.5033 0 TD
(P)Tj
/F6 1 Tf
.7833 0 TD
(<)Tj
/F4 1 Tf
.7142 0 TD
[(0.0001,)-334.9(**)]TJ
/F9 1 Tf
4.3371 0 TD
(P)Tj
/F6 1 Tf
.7776 0 TD
(<)Tj
/F4 1 Tf
.72 0 TD
[(0.00010.01,)-337(*)]TJ
/F9 1 Tf
6.0823 0 TD
(P)Tj
/F6 1 Tf
.7833 0 TD
(<)Tj
/F4 1 Tf
.72 0 TD
(0.010.05.)Tj
/F1 1 Tf
9.9626 0 0 9.9626 59.7543 102.1606 Tm
[(Resu)-10.9(lts)]TJ
/F8 1 Tf
0 -1.673 TD
[(USS)-10(Cs)-335.5(fail)-335.7(to)-333.6(s)-6.5(timulate)-337(all)-7.8(ogeneic)-340.3(T-lympho)-11.4(cytes)]TJ
/F4 1 Tf
9.843 0 0 9.843 69.7323 67.6346 Tm
[(The)-270.6(level)-273(of)-267.1(the)-269.5(immune)-269.6(response)-269.9(provoked)-269.1(by)-267.1(USSCs)-271.8(as)]TJ
-1.0137 -1.1116 TD
[(stimulators)-268(of)-261.4(allogeneic)-263.7(T-lymphocytes)-261(was)-264.3(tested.)-265.9(PHA)-258.3(or)]TJ
25.5097 67.9127 TD
[(monocyte-derived)-369.3(i)21.8(m)15(m)20.7(a)20.8(t)21.8(u)16.2(r)22(e)-273(D)21.1(C)20.9(s)-271.6(\()16.2(i)21.8(D)21.1(C)15.2(s)22.1(\))-271.8(w)21.1(e)20.8(r)16.2(e)-273(u)21.9(s)22.1(e)15(d)-271.8(a)20.8(s)-271.6(s)22.1(t)16.1(i)21.8(m)20.7(u)21.9(-)]TJ
0 -1.1174 TD
-.0216 Tc
[(lat)-5.5(o).3(rs)-195.3(in)-189.7(co)-5.4(ntr)-5.4(o).3(l)-189.9(c)-.8(u)-5.4(ltu)-5.4(res)-5.2(.)-186.3(O)-6.3(n).3(ly)-195.5(a)-190.9(s).5(li)-5.5(ght)-195.6(incr)-5.4(eas)-5.2(e)-190.9(i).2(n)-189.7(T)-6.6(-ly)-5.4(m)-.9(pho)-5.4(cyt)-5.6(e)]TJ
0 -1.1116 TD
[(pro)-5.4(lif)-5.4(era)-6.6(tio)-5.4(n)-178.2(w)-.5(as)-183.8(obs)-5.2(erv)-5.4(e)-.8(d)-178.2(w)-.5(h)-5.4(e)-.8(n)-178.2(U)-6.3(SSCs)-183.8(w)-.5(er)-5.4(e)-179.4(u).3(s)-5.2(e)-.8(d)-178.2(a)-.8(s)-183.8(s).5(ti)-5.5(mul)-5.6(a)-.8(tor)]TJ
T*
-.0205 Tc
[(cells)-228.8(as)-228.8(c)-5.5(o)1.4(mpared)-228.9(w)-5.2(ith)-228.9(the)-230.1(n)1.4(e)-5.5(g)1.4(at)]TJ
12.4237 0 TD
-.0217 Tc
[(ive)-231.3(c)-.9(o)-5.5(n).2(tr)-5.5(ol)-230.3(\(F)-5.4(ig.)-232.5(1).2(A)-6.4(\)).3(.)-232.5(T)-1(his)-235.7(l).1(ack)]TJ
-12.4237 -1.1116 TD
[(of)-402.9(sti)-5.6(m)-1(ula)-6.7(t).1(or)-402.9(act)-5.7(ivi)-5.7(t).1(y)-397.2(i)-5.6(s)-397(c)-.9(o)-5.5(n).2(si)-5.6(ste)-6.7(n).2(t)-397.3(w)-6.4(ith)-402.9(the)-6.7(i).1(r)-397.1(p)-5.5(hen)-5.5(o).2(ty)-5.5(pe;)-403.1(like)]TJ
0 -1.1174 TD
-.02 Tc
[(BM-MSCs,)-397.8(USSCs)-401(d)1.9(o)-401.2(not)-401.4(express)-395.3(M)-4.7(HC)-396.5(c)-5(l)1.8(ass)-395.3(I)-3.8(I)-395.4(m).7(olec)-5(ules,)]TJ
0 -1.1116 TD
.2372 Tc
[(C)258.1(D)258.3(8)253.4(0)0(a)258(n)253.4(dC)258.1(D)252.5(8)259.1(6[)253.4(8)259.1(])259.2(.)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 310.8472 637.455 Tm
0 Tc
[(USSC)-9(s)-330.8(inhi)-7.8(bit)-331.8(DC)-9.4(-induce)-7.3(d)-330(T-ly)-8(mphocyt)-8.5(e)]TJ
0 -1.2007 TD
[(prolife)-8.4(ration)-340.2(in)-334(a)-335.7(dose-dep)-9.1(endent)-339.2(manner)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 607.6912 Tm
[(The)-351.2(effect)-350(of)-353.5(USSCs)-352.5(on)-353.5(T-lymphocyte)-352.3(proliferation)-356.9(was)]TJ
-1.0137 -1.1116 TD
[(evaluated)-297.1(by)-295.9(mixing)-296.1(different)-300.4(amounts)-298(of)-295.9(USSCs)-294.9(and)-298.2(PBLs)]TJ
0 -1.1174 TD
[(in)-255.8(the)-258(presence)-266.7(of)-255.6(allogeneic)-263.7(DCs)-258.4(as)-262.4(stimulators.)-260(PBLs)-259.7(were)]TJ
0 -1.1116 TD
[(always)-326.5(stimulated)-331.7(with)-328.1(DCs)-327.6(at)-326(a)-324.8(ratio)-329.4(of)-324.7(15:1,)-330.5(because)-329(this)]TJ
T*
[(ratio)-381.3(induces)-390.1(optimal)-384.9(T-cell)-384.7(stimulation.)-387(As)-383(shown)-380.6(in)-382.5(Fig.)]TJ
0 -1.1174 TD
[(1B,)-398.3(a)-399.7(huge)-403(reduction)-404.1(in)-399.7(T-lymphocyte)-398.3(proliferatio)-10.2(n)-398.5(i)0(s)-399.6(ob-)]TJ
0 -1.1116 TD
[(served)-590.4(when)-593.9(DC-stimulated)-596.1(PBLs)-593.8(were)-595.1(cultured)-593.1(in)-589.8(the)]TJ
T*
[(presence)-353.1(of)-347.8(USSCs.)-350.2(Titration)-346.8(studies)-348.6(show)-350.7(that)-351.4(this)-344.3(inhibi-)]TJ
0 -1.1174 TD
[(tory)-361.6(effect)-367.3(is)-359.2(dose)-362.5(dependent)-364.9(\(Fig.)-364.9(1B\).)-364.8(Optimal)-362.7(inhibition)]TJ
0 -1.1116 TD
[(was)-333.4(observed)-339.2(for)-331.5(one)-332.8(USSC)-334.3(for)-337.3(every)-336.1(DC.)]TJ
1.0137 -1.1116 TD
-.0217 Tc
[(T)-21.7(o)-313.1(un)-5.5(rave)-6.7(l)-218.8(t)-5.7(he)-219.8(k)-5.5(i).1(net)-5.6(i).1(cs)-224.2(of)-224.4(USS)-5.4(C)-219.7(i)-5.6(nhib)-5.5(iti)-5.7(on)-224.4(on)-218.6(T)-6.7(-).3(ly)-5.5(mpho)-5.5(cyt)-5.7(e)]TJ
-1.0137 -1.1116 TD
-.0215 Tc
[(pro)-5.3(lif)-5.3(era)-6.5(tio)-5.3(n,)-295.6(US)-5.2(SCs)-298.9(w)-.4(er)-5.3(e)-294.5(a)-.7(d)-5.3(d).4(ed)-299.1(at)-299.2(var)-5.3(i).3(ou)-5.3(s)-293.1(t).3(i)-5.4(m)-.8(e)-294.5(p)-5.3(oin)-5.3(t).3(s:)-299.2(0,)-295.6(4)-5.3(8).4(,)]TJ
0 -1.1174 TD
[(72,)-324.5(9)-5.4(6).3(,)-324.5(o).3(r)-327.9(120)-166.7(h)-5.4(,)-324.5(aft)-5.5(e)-.8(r)-322.2(M)-6.3(LR)-323.2(in)-5.4(iti)-5.6(ati)-5.5(o).3(n)-322.2(\()-5.4(Fig)-5.4(.)-318.8(1)-5.4(C)-.7(\).)-324.5(O)-6.3(p).3(ti)-5.6(mal)-322.3(s)-5.2(up-)]TJ
0 -1.1116 TD
[(pre)-6.5(s).6(s)-5.1(i).3(on)-512.2(o)-5.3(f)-512.1(T-l)-5.4(y).4(mp)-5.3(hocy)-5.3(t).3(e)-513.3(p)-5.3(rol)-5.4(i).3(fe)-6.5(rati)-5.4(on)-512.2(w)-6.2(a)-.7(s)-512(o).4(b)-5.3(s).6(er)-5.3(ved)-517.9(w)-.4(hen)]TJ
T*
-.0217 Tc
[(USS)-5.4(C)-.8(s)-201.1(w)-.6(ere)-202.5(a)-.9(dd)-5.5(ed)-195.6(a)-6.7(t)-195.7(th)-5.5(e)-196.8(b).2(e)-6.7(g).2(inn)-5.5(i).1(ng)-201.3(of)-201.3(the)-202.5(c)-.9(ult)-5.6(u).2(re)-6.7(.)-197.9(I).3(nt)-5.6(ere)-6.7(s).4(ti)-5.6(ngl)-5.7(y,)]TJ
0 -1.1174 TD
-.0215 Tc
[(whe)-6.5(n)-264.5(USS)-5.2(C)-.6(s)-264.3(w)-.4(er)-5.3(e)-265.7(a)-.7(dd)-5.3(ed)-264.5(120)-172.3(h)-264.5(a)-.7(ft)-5.4(er)-264.5(MLR)-265.5(i)-5.5(nit)-5.4(i).3(ati)-5.4(o).4(n)-264.5(\().5(i)-5.5(m)-.8(pl)-5.5(ying)]TJ
0 -1.1116 TD
[(onl)-5.5(y)-235.8(4)-5.4(8)-166.7(h)-235.8(o)-5.4(f)-235.8(co)-5.4(cul)-5.5(t).2(ur)-5.4(e)-237(o).3(f)-241.5(U)-.5(SS)-5.3(Cs)-241.4(wit)-5.5(h)-235.8(ly)-5.4(mpho)-5.4(cyt)-5.5(e)-.8(s)-241.4(e)-.8(nga)-6.6(ged)-241.6(f).4(or)]TJ
T*
[(the)-352.2(M)-6.3(LR\))-351(s)-5.2(til)-5.5(l)-351.1(a)-352.2(mar)-5.4(k).3(ed)-351(s)-5.2(upp)-5.4(res)-5.2(s).5(io)-5.4(n)-351(o).3(f)-351(T)-.9(-)-5.4(l).2(ym)-6.6(phoc)-6.6(yte)-352.2(p)-5.4(rol)-5.5(i).2(f-)]TJ
0 -1.1174 TD
-.0178 Tc
[(er)4.2(at)4(i)4(o)-1.6(n)-180.2(c)3(ou)4.1(l)4(d)-180.2(be)-181.3(o)4.1(b)-1.6(s)4.3(e)3(rv)4.1(ed)-185.9(\()]TJ
/F9 1 Tf
10.1889 0 TD
0 Tc
(P)Tj
/F6 1 Tf
.7603 0 TD
(=)Tj
/F4 1 Tf
.6969 0 TD
-.0215 Tc
[(0)-5.3(.)-1.9(058)-5.3(\),)-186.2(in)-5.3(dica)-6.5(tin)-5.3(g)-183.9(t).3(h)-5.3(a)-.7(t)-184(U)-.4(S)-5.2(S).6(Cs)-189.4(ar)-5.3(e)]TJ
-11.6461 -1.1116 TD
[(ver)-5.3(y)-293.3(ef)-5.2(c)-.7(ien)-5.3(t)-293.4(in)-293.3(the)-294.5(s)-5.1(uppr)-5.3(es)-5.1(sio)-5.3(n)-287.5(o)-5.3(f)-293.3(T-l)-5.5(y).4(mph)-5.3(o).4(cyt)-5.4(e)-294.5(pro)-5.3(lif)-5.3(erat)-5.4(ion)]TJ
T*
[(and)-264.6(not)-264.7(in)-264.6(pre)-6.6(v).3(ent)-5.5(i).2(ng)-264.6(T-c)-6.6(e)-.8(ll)-264.7(act)-5.5(iva)-6.6(tion)-5.4(.)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 310.8472 366.8597 Tm
0 Tc
[(Regula)-8.5(tory)-337.1(T-cell)-336.3(popul)-9.5(ation)-337.4(is)-334.8(not)-339.2(increase)-8.1(d)]TJ
0 -1.195 TD
[(in)-334(MLR)]TJ
/F6 1 Tf
3.3802 0 TD
(+)Tj
/F8 1 Tf
.717 0 TD
[(USS)-10(C)-334.8(cultures)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 337.096 Tm
[(Treg)-432.9(ce)18.5(ll)20.6(s)-352.2(a)0(r)19.7(e)-353.6(id)20.7(en)19.7(ti)20.6(e)19.8(d)-352.4(b)0(y)-342(t)0(h)20.7(e)0(i)19.5(r)-352.4(hi)20.7(gh)-342(le)19.6(ve)19.7(l)-352.6(o)0(f)-342(C)0(D)18.9(2)0(5)-336.3(a)0(n)19.7(d)]TJ
-1.0137 -1.1116 TD
[(CD)18.9(4)-323.6(e)0(x)19.7(p)0(r)20.9(e)0(s)19.9(s)0(i)15.1(o)0(n)20.8(,)-320.2(wh)20(il)14.8(e)-324.8(a)0(l)19.6(s)0(o)-313(e)0(x)19.7(p)0(r)20.9(e)0(s)19.9(s)0(i)15.1(n)0(g)-313.2(l)0(o)20.7(w)-324.5(le)19.5(ve)19.7(ls)-313.2(of)-313.2(CD)18.9(12)20.8(7)]TJ
0 -1.1174 TD
[([3)20.9(0])20.9(.)-279.9(T)0(r)19.7(e)0(g)-274.1(c)0(e)18.5(l)0(l)20.6(s)-277.4(ar)19.7(e)-284.5(f)0(u)20.9(r)0(t)20.7(h)0(e)19.7(r)-283.3(de)19.7(n)21(ed)-268.3(by)-272.9(ex)19.7(pr)20.9(es)19.9(si)15.1(on)-267.1(of)-272.9(th)20.7(e)-278.7(f)0(o)20.9(r)0(k)20.9(-)]TJ
0 -1.1116 TD
[(he)19.7(ad)-418.1(fa)19.7(mi)19.5(ly)-417(tr)15(an)19.7(sc)19.9(ri)20.7(pt)20.7(io)20.7(n)-427.3(f)0(a)14(c)0(t)19.5(o)0(r)-416.9(F)0(O)20.1(X)0(P)20.1(3)-427.3(\(f)15.1(or)20.9(kh)20.8(ea)18.5(d)-427.3(b)0(o)20.8(x)-427.3(p3)20.8(\))]TJ
T*
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T*
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