PubMed

Foods. 2024 Dec 20;13(24):4133. doi: 10.3390/foods13244133.ABSTRACTTo gain a deeper understanding of the mechanisms by which cushioning packaging preserves the quality of Chinese olive fruits during cold chain transportation and extends their shelf life, this study simulated cold chain conditions and investigated the effects of cushioning packaging on the physiology, antioxidant capacity, and secondary metabolites of fruits during a 20-day shelf life. The results indicated that the decay rate in cushioning-packaging-treated fruit was 75% lower than that in the unbuffered packaging fruit at day 20 of shelf life. Simultaneously, cushioning packaging treatment mitigated the damage severity of the cell membrane structure and kept the cell membrane permeability at a low level, which was 15.34% lower than that in the unbuffered packaging fruit at day 20 of shelf life. Additionally, cushioning packaging effectively restrained the increases in malondialdehyde (MDA) content and alleviated the decline in chlorophyll and total flavonoid contents. It kept a balance among reactive oxygen species (ROS), antioxidant levels, and antioxidant enzyme activities, thereby reducing mechanical-damage-induced decay rates in Chinese olive fruits during the shelf life. Furthermore, metabolome analysis of Chinese olives during the shelf life was performed comparing those without buffered packaging to those with buffered packaging. The metabolome analysis found that the flavonoid biosynthetic pathway exhibited a higher accumulation of chrysin, neohesperidin, naringenin chalcone, sakuranetin, quercetin, catechin, and naringenin metabolites in cushion-packaging treatment compared to those without cushioning treatment. Furthermore, within the phenylalanine metabolic pathway, the accumulation of phenylalanine, p-coumaraldehyde, p-coumaric acid, coniferin and caffeoyl quinic acid metabolites was significantly higher in buffered-packaging groups compared to those without buffering. Together, these findings suggest that cushioning packaging can effectively sustain the integrity of cell membranes and enhance the shelf-life quality of Chinese olive fruits by regulating the balance of ROS and mitigating oxidative stress during cold chain transportation.PMID:39767075 | DOI:10.3390/foods13244133

Foods. 2024 Dec 18;13(24):4104. doi: 10.3390/foods13244104.ABSTRACTChlorogenic acid (CGA), a polyhydroxy phenolic acid, has been extensively studied for its antimicrobial properties. Staphylococcus aureus (S. aureus) threatens food safety by forming biofilms. This study aimed to investigate the mechanism of CGA against S. aureus and its biofilm. The anti-bacterial activity of CGA was assessed using crystal violet staining, TEM, SEM, a CLSM, and using metabolomics and molecular docking to elucidate the mechanism. The results indicated that the minimum inhibitory concentration of CGA against S. aureus was 2.5 mg/mL. CGA disrupts the integrity of bacterial cell membranes, leading to increased hydrophobicity, morphological changes, scattering, and reduced spreading. This disruption decreases biofilm adhesion and bacterial count. Metabolomics and molecular docking analyses revealed that CGA down-regulates key amino acids. It forms hydrogen bonds with penicillin-binding protein 4 (PBP4), Amidase, glutamate synthetase B, and glutamate synthetase A. By inhibiting amino acid metabolism, CGA prevents biofilm formation. CGA interacts with amino acids such as aspartic acid, glutamine, and glutamate through hydroxyl (-OH) and carbonyl (-C=O) groups. This interaction reduces cell viability and biofilm cohesion. The novel findings of this study, particularly the extension of the shelf life of pasteurized milk by inhibiting S. aureus growth, highlight the potential of CGA as a promising anti-biofilm strategy and preservative in the dairy industry.PMID:39767046 | DOI:10.3390/foods13244104

Foods. 2024 Dec 17;13(24):4076. doi: 10.3390/foods13244076.ABSTRACTThis study investigated the effects of three different single-strain probiotics Lactiplantibacillus plantarum XD117, Lacticaseibacillus paracasei LC-37, and Lacticaseibacillus rhamnosus LGG, on the quality of hempseed fermented milk. The main findings were that adding probiotics increased the inhibition rate of α-glucosidase and pancreatic lipase in hempseed fermented milk significantly. Non-targeted metabolomic correlation analysis results confirmed that 14 substances, including three flavonoids, six amino acids and their derivatives, and five short peptides, were positively correlated with the hypoglycemic and hypolipidemic activities of hempseed fermented milk. Furthermore, a total of 59 volatile flavor compounds were identified, including aldehydes, alcohols, ketones, acids, and esters, and the role mapping of different probiotic communities was provided. These results can guide the development of hempseed fermented milk with unique flavor, rich probiotic content, and significant functional characteristics.PMID:39767016 | DOI:10.3390/foods13244076

Foods. 2024 Dec 16;13(24):4058. doi: 10.3390/foods13244058.ABSTRACTHyperlipidemia poses significant risks for cardiovascular diseases, with emerging evidence underscoring the critical role of gut microbiota in metabolic regulation. This study explores Lactobacillus casei CAAS36, a probiotic strain with promising cholesterol-lowering capabilities, assessing its impact on hyperlipidemic hamsters. Utilizing 1H NMR-based metabolomics and 16S rRNA gene sequencing, we observed that L. casei CAAS36 treatment not only altered metabolic pathways but also reshaped gut microbiota composition. Notably, the treatment restored the balance between Firmicutes and Bacteroidetes and significantly increased the abundance of propionate-producing Muribaculaceae. Metabolically, L. casei CAAS36 administration led to the normalization of key lipid markers, including reductions in total cholesterol, LDL-C, and triglycerides (29.9%, 29.4% and 32.6%), while enhancing the protective HDL-C levels. These effects were accompanied by significant increases in beneficial metabolites such as propionate and succinate, which are known for their roles in preventing metabolic disorders. These findings highlight the dual regulatory effects of L. casei CAAS36 on the metabolic profile and gut microbiota, suggesting a substantial potential for this probiotic in the management of hyperlipidemia and possibly other metabolic diseases. Future applications may include its use as a natural therapeutic agent in clinical settings, aiming to reduce reliance on conventional pharmaceuticals and their associated side effects.PMID:39767000 | DOI:10.3390/foods13244058

Foods. 2024 Dec 16;13(24):4055. doi: 10.3390/foods13244055.ABSTRACTArsenic is a common toxic heavy metal contaminant that is widely present in the ocean, and seaweeds have a strong ability to concentrate arsenic, posing a potential risk to human health. This study first analyzed the arsenic content in two different seaweeds and then used an innovative method to categorize the seaweeds into low-arsenic and high-arsenic groups based on their arsenic exposure levels. Finally, a non-targeted metabolomic analysis based on mass spectrometry was conducted on seaweed from different arsenic exposure groups. The results indicated that as the arsenic concentration increased in the seaweeds, linolenic acid, tyrosine, pheophorbide a, riboflavin, and phenylalanine were upregulated, while arachidonic acid, eicosapentaenoic acid (EPA), betaine, and oleamide were downregulated. The following four key metabolic pathways involving unsaturated fatty acids and amino acids were identified: isoquinoline alkaloid biosynthesis, tyrosine metabolism, phenylalanine metabolism, and riboflavin metabolism. The identification of biomarkers and the characterization of key metabolic pathways will aid in the selection and breeding of low-arsenic-accumulating seaweed varieties, providing insights into the metabolic and detoxification mechanisms of arsenic in seaweeds.PMID:39766997 | DOI:10.3390/foods13244055

Foods. 2024 Dec 12;13(24):4025. doi: 10.3390/foods13244025.ABSTRACTSweet corn is a globally important food source and vegetable renowned for its rich nutritional content. However, post-harvest quality deterioration remains a significant challenge due to sweet corn's high sensitivity to environmental factors. Currently, low-temperature storage is the primary method for preserving sweet corn; however, the molecular mechanisms involved in this process remain unclear. In this study, kernels stored at different temperatures (28 °C and 4 °C) for 1, 3, and 5 days after harvest were collected for physiological and transcriptomic analysis. Low temperature storage significantly improved the PPO and SOD activity in sweet corn kernels compared to storage at a normal temperature. A total of 1993 common differentially expressed genes (DEGs) were identified in kernels stored at low temperatures across all three time points. Integrated analysis of transcriptomic and previous metabolomic data revealed that low temperature storage significantly affected flavonoid biosynthesis. Furthermore, 11 genes involved in flavonoid biosynthesis exhibited differential expression across the three storage periods, including CHI, HCT, ANS, F3'H, F3'5'H, FLS, and NOMT, with Eriodictyol, Myricetin, and Hesperetin-7-O-glucoside among the key flavonoids. Correlation analysis revealed three AP2/ERF-ERF transcription factors (EREB14, EREB182, and EREB200) as potential regulators of flavonoid biosynthesis during low temperature treatment. These results enhance our understanding of the mechanisms of flavonoid synthesis in sweet corn kernels during low-temperature storage.PMID:39766968 | DOI:10.3390/foods13244025

Foods. 2024 Dec 12;13(24):4018. doi: 10.3390/foods13244018.ABSTRACTCitrus reticulata 'Chachi' (CRC), recognized for its considerable edible and medicinal significance, is a valuable source of metabolites beneficial to human health. This research investigates the metabolic distinctions and antioxidant properties across four different parts of CRC, using multivariate statistical analysis to interpret metabolomic data and network pharmacology to identify potential antioxidant targets and relevant signaling pathways. The results indicate considerable metabolic differences in different parts of the sample, with 1622 metabolites showing differential expression, including 816 secondary metabolites, primarily consisting of terpenoids (31.02%) and flavonoids (25.22%). The dried mature citrus peel (CP) section demonstrates the highest level of total phenolics (6.8 mg/g), followed by the pulp without seed (PU) (4.52 mg/g), pulp with seed (PWS) (4.26 mg/g), and the seed (SE) (2.16 mg/g). Interestingly, targeted high-performance liquid chromatography of flavonoids reveals the highest level of nobiletin and tangeretin in CP, whereas PU has the highest level of hesperidin, narirutin, and didymin. Furthermore, all four sections of CRC exhibit robust antioxidant properties in in vitro assessments (CP > PU > PWS > SE). Lastly, the network pharmacology uncovered potential antioxidant mechanisms in CRC. This research offers deeper insights into the development and utilization of byproducts in the CRC processing industry.PMID:39766961 | DOI:10.3390/foods13244018

Fitoterapia. 2025 Jan 5:106378. doi: 10.1016/j.fitote.2025.106378. Online ahead of print.ABSTRACTGenus Acacia comprises around 1500 species. They are widely used to treat inflammation as well as bacterial and fungal infections as they are enriched in phytochemicals, especially phenolics. The aim of this study was to evaluate the antibacterial activity of leaves' methanolic extracts of twelve Acacia species growing in Egypt against Vibrio parahaemolyticus, Salmonella enterica, Listeria monocytogens, Klebsiella pnemoniae, Bacillus aquimaris, Bacillus subtilis, and Escherichia coli. These species are Acacia nilotica (wild and cultivated), Acacia seyal, Acacia auriculiformis, Acacia saligna, Acacia xanthophloea, Acacia tortilis subsp. raddiana (Gabal Elba and Aswan), Acacia tortilis, Acacia laeta (wild and cultivated), and Acacia albida. Furthermore, to study the metabolomic composition and variation among these species using ultra-high-performance liquid chromatography-electrospray ionization quadrupole time of flight mass spectrometry (UHPLC-q-tof-ESI-MS) coupled with multivariate statistical analysis and correlate it to the antibacterial potential. Results showed that Acacia nilotica (AN) has superior antibacterial activity over the other species. In addition, it exhibited a distinct segregation in Principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA). Full profiling of AN using UHPLC-ESI-q-tof-MS revealed 42 phenolics mainly catechins. It was further subjected to bio-guided fractionation and revealed the presence of methyl gallic acid, gallic acid, catechin gallate, and digallate isomers in its most bioactive fraction. These compounds were identical to the compounds annotated as VIPs and were responsible for the segregation of AN in both PCA and PLS-DA analyses. Hence, this study sheds light on the use of chemometrics as an early tool for the detection of bioactive compounds.PMID:39765316 | DOI:10.1016/j.fitote.2025.106378

Ecotoxicol Environ Saf. 2025 Jan 6;289:117646. doi: 10.1016/j.ecoenv.2024.117646. Online ahead of print.ABSTRACTIn this study, male Sprague-Dawley (SD) rats were exposed to bisphenol S (BPS) at environmentally relevant concentrations to investigate its reproductive toxicity and evaluate its effects on the gut-blood-testicular axis. After 28 days of exposure to BPS (0.05 and 20 mg/kg), the results showed a reduction in weight gain and the induction of reproductive toxicity in male rats, including decreased sperm parameters, lower sperm viability, and increased abnormal sperm density and mortality. These observations were made by counting with a hemocytometer under the optical microscope. 16S rRNA and untargeted metabolomic elucidated potential impacts on the gut-blood-testicular axis: BPS impaired the physical barrier, evoked inflammation, and resulted in dysbiosis of the gut microbiota. Additionally, BPS altered serum metabolites, including phosphatidic acid and diacylglycerol, which are involved in Fc gamma R-mediated phagocytosis and linked to inflammation. Furthermore, histopathological analysis, western blot (WB), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence results showed that exposure to BPS led to testicular damage, inflammation, activation of the p38 and ERK MAPK pathways, and disruption of the blood-testis barrier (BTB). Collectively, these findings indicate that BPS impair the intestinal health, disrupt gut microbiome, and ultimately lead to reproductive dysfunction through the gut-blood-testicular axis.PMID:39765121 | DOI:10.1016/j.ecoenv.2024.117646

Talanta. 2025 Jan 4;286:127536. doi: 10.1016/j.talanta.2025.127536. Online ahead of print.ABSTRACTMatrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has become a robust tool for analyzing a variety of biomacromolecules. However, the strong background interference produced by conventional organic matrices hinders the detection of small molecule analytes, which restricts the widespread application of MALDI-MS in metabolomics studies. Consequently, developing new organic matrices is urgently needed to overcome these issues. In this study, 1,4-Dioxo-1,2,3,4-tetrahydrophthalazine-6-carboxylic acid (DTCA) was firstly employed as a new matrix for MALDI-MS to enhance the detection of low molecular weight compounds because of its strong UV absorption, less matrix background interference, high ionization efficiency for metabolites, and good reproducibility. Considering these advantages, DTCA was used to analyze endogenous metabolites in the serum samples of imidacloprid (IMI)-exposed mice via MALDI-MS in positive ion mode. By combining with machine learning, the differentiation between imidacloprid-exposed mice and control mice was successfully achieved, and 39 metabolites were estimated as potential biomarkers. Additionally, potentially disrupted metabolic pathways were revealed. These results indicate that DTCA, as a new and powerful matrix in positive ion mode, has great potential for applications in the detection of small molecules.PMID:39765079 | DOI:10.1016/j.talanta.2025.127536

J Environ Manage. 2025 Jan 6;374:123969. doi: 10.1016/j.jenvman.2024.123969. Online ahead of print.ABSTRACTThe increasing contamination of ecosystems with heavy metals (HMs) due to industrial activities raises significant jeopardies to environmental health and human well-being. Addressing this issue, recent advances in the field of bioremediation have highlighted the potential of plant-associated microbiomes and genetically engineered organisms (GEOs) to mitigate HMs pollution. This review explores recent advancements in bioremediation strategies for HMs detoxification, with particular attention to omics technologies such as metagenomics, metabolomics, and metaproteomics in deepening the understanding of microbial interactions and their potential for neutralizing HMs. Additionally, Emerging strategies and technologies in GEOs and microorganism-aided nanotechnology have proven to be effective bioremediation tools, particularly for alleviating HM contamination. Despite the promising strategies developed in laboratory settings, several challenges impede their practical application, including ecological risks, regulatory limitations, and public concerns regarding the practice of genetically modified organisms. A comprehensive approach that involves interdisciplinary research is essential to enhance the efficacy and safety of bioremediation technologies. This approach should be coupled with robust regulatory frameworks and active public engagement to ensure environmental integrity and societal acceptance. This review underscores the importance of developing sustainable bioremediation strategies that align with ecological conservation goals and public health priorities.PMID:39765072 | DOI:10.1016/j.jenvman.2024.123969

Phytomedicine. 2025 Jan 1;136:156361. doi: 10.1016/j.phymed.2025.156361. Online ahead of print.ABSTRACTBACKGROUND: Artemisia spp. have been used for millennia in traditional medicine to treat a variety of ailments, including malaria. Extracts of Artemisia afra and A. annua remain widely used throughout Africa for healthcare purposes, notably to prevent and/or treat malaria. However, the modes of action of these plant extracts remain unclear, with contradictory reports regarding the presence and role of artemisinin in both plants.PURPOSE: The aim of this study was to identify differences in the antimalarial mode of action of A. afra and A. annua by measuring their phenolic profiles and comparing their effect on parasite metabolism in vitro.METHODS: In this work, we analyzed the phenolic profile of A. afra and A. annua extracts through high-performance liquid chromatography (HPLC), detected and quantified artemisinin through HPLC and mass spectrometry (MS), and performed comparative HPLC-MS metabolomic analysis on in vitro-cultured Plasmodium falciparum trophozoites to elucidate the potential modes of action of these plant extracts.RESULTS: A. afra contained only trace amounts of artemisinin and elicited a different parasite metabolic response compared to A. annua, which contained significantly more artemisinin and correlated closely with the parasite response profile elicited by purified artemisinin. A. annua impacted parasite glutathione metabolism in agreement with the established redox activity of artemisinin, while A. afra had an effect on lipid precursors.CONCLUSIONS: This study reveals that A. afra and A. annua have divergent effects on Plasmodium falciparum metabolism and provides support for ongoing efforts exploring the use of A. afra for the treatment of malaria.PMID:39765035 | DOI:10.1016/j.phymed.2025.156361

Vet Microbiol. 2024 Dec 31;301:110365. doi: 10.1016/j.vetmic.2024.110365. Online ahead of print.ABSTRACTBluetongue (BT) is a viral vector borne disease primarily affecting ruminants such as sheep, cattle, and goats. On 3 September 2023, the Netherlands reported the first case of bluetongue virus serotype 3 (BTV-3/NET2023)), after being BTV free for eleven years. Vaccination with inactivated BT vaccines for serotype 3 has been applied in the Netherlands since May 2024. Nonetheless, in late June/July 2024, BTV-3 re-emerged and spread over large parts of Europe. In October 2024, BTV-12 was identified by follow-up diagnostics after a BTV-3 vaccinated sheep with signs of BT was tested positive for BTV but negative for serotype 3. This marks a significant event, as BTV-12 had never been reported in Europe. Screening of farms in close proximity to the sheep farm and retrospective analysis of samples from clinically affected animals that were panBTV PCR positive resulted in the detection of nine BTV-12 affected farms in total. The emergence of BTV-12 in the Netherlands raises important questions about the route of introduction of BT in the Netherlands and mechanisms of viral spread of this specific serotype. Possible adaptation of new BTV serotypes to the European climatic and husbandry conditions prompts reconsideration of prevention, surveillance, and control strategies in relation to changing ecological conditions and vector dynamics. The initial findings, respective studies as well as the initial attempts to trace the origin of BTV-12/NET2024 are described.PMID:39765008 | DOI:10.1016/j.vetmic.2024.110365

Syst Appl Microbiol. 2025 Jan 3;48(1):126579. doi: 10.1016/j.syapm.2025.126579. Online ahead of print.ABSTRACTSix novel Bifidobacterium strains H1HS16NT, Bin2N, Hma3N, H6bp22N, H1HS10N, and H6bp9N, were isolated from the honey stomach of Apis mellifera. Cells are Gram-positive, non-motile, non-sporulating, facultatively anaerobic, and fructose 6-phosphate phosphoketolase-positive. Optimal growth conditions occur at 37 °C in anaerobiosis in MRS medium added with 2 % fructose and 0.1 % L-cysteine. The 16S rRNA gene sequences analysis revealed clustering with Bifidobacterium species found in honeybees. Strains Hma3N, H6bp22N, and H1HS16NT showed significant similarity to Bifidobacterium polysaccharolyticum JCM 34588T, with an average similarity of 99.63 %. In contrast, strains Bin2N, H1HS10N, and H6bp9N were closely related to Bifidobacterium apousia JCM 34587T, with an average similarity of 99.22 %. Moreover, strains Hma3N and H6bp22N exhibited ANI values of 96.65 % and 96.53 % when compared to Bifidobacterium polysaccharolyticum JCM 34588T, while strains H1HS16NT, Bin2N, H6bp9N, and H1HS10N revealed ANI values of 94.18 %, 94.33 %, 94.22 %, and 95.50 % respectively when compared to B. apousia JCM 34587T. dDDH analysis confirmed that strains Hma3N and H6bp22N belong to B. polysaccharolyticum, whereas strains H1HS16NT, Bin2N, H6bp9N, and H1HS10N represent a novel species. The peptidoglycan of the novel species is of the A4α type (L-Lys-D-Asp). The main cellular fatty acids of the type strain H1HS16NT are C16:0, C14:0, C19:0 cyclo ω9c, and C18:1 ω9c. The DNA G + C content of the type strain is 60.8 mol%. Genome analyses of the strains were also conducted to determine their biosynthesis-related gene clusters, probiotic features, and ecological distribution patterns. Phenotypic and genotypic characterization show that strain H1HS16NT is distinct from the type strains of other recognized Bifidobacterium species. Thus, Bifidobacterium kimbladii sp. nov. (H1HS16NT = DSM 115187T = CCUG 76695T) is proposed as a novel Bifidobacterium species.PMID:39764984 | DOI:10.1016/j.syapm.2025.126579

J Hazard Mater. 2024 Dec 31;486:137066. doi: 10.1016/j.jhazmat.2024.137066. Online ahead of print.ABSTRACTPolylactic acid (PLA) microplastics (MPs) and lead (Pb) co-contamination, an emerging co-contamination, may profoundly impact plant growth. We aimed to evaluate the effects of PLA-MPs and Pb on buckwheat growth and physiology and to elucidate the underlying molecular mechanisms through an integrated transcriptomic and metabolomic approach. PLA-MPs alone reduced buckwheat biomass by 26.0 %, while combined exposure to Pb and PLA-MPs (PLA-Pb) further exacerbated morphological impairments, decreasing biomass by 43.1 % and 50.4 % compared to the control. Antioxidant enzyme activities increased under Pb and PLA-Pb treatments. The analysis revealed 536 differentially expressed metabolites (DEMs) and 3229 differentially expressed genes (DEGs) in PLA-Pb vs. control, 168 DEMs and 1555 DEGs in PLA-Pb vs. PLA, and 196 DEMs and 4057 DEGs in PLA-Pb vs. Pb. Key DEGs involved in lignin biosynthesis, including caffeoyl-CoA-O-methyltransferase, cinnamoyl-CoA reductase, and catechol-O-methyltransferase, were upregulated, suggesting that buckwheat mitigates toxicity by enhancing cell wall modification. Similarly, DEGs and DEMs linked to jasmonate biosynthesis were enriched in the alpha-linolenic acid metabolic pathway, including allene oxide synthase, allene oxide cyclase, and 12-oxophytodienoate reductase, as well as methyl jasmonate. These results suggest that buckwheat counters PLA-MPs-Pb toxicity by enhancing oxidative stress responses and upregulating the synthesis of lignin and unsaturated fatty acids. In conclusion, this study provides novel insights into the molecular mechanisms of plant detoxification under PLA-MPs-Pb co-exposure, highlighting the need for ecological risk assessment of combined microplastic and heavy metal pollution.PMID:39764956 | DOI:10.1016/j.jhazmat.2024.137066

Mult Scler Relat Disord. 2025 Jan 6;94:106250. doi: 10.1016/j.msard.2024.106250. Online ahead of print.ABSTRACTBACKGROUND: Radiologically Isolated Syndrome (RIS) characterized by abnormalities on MRI that do not manifest as clinical symptoms of Multiple Sclerosis (MS) but raise suspicion for MS. Considering that RIS often evolves into MS, various diagnostic criteria have been established, and each suggested biomarker warrants thorough consideration and discussion. In this study, metabolomic profiling of body fluids of patients who were being followed up with a pre-diagnosis of RIS or MS and had not yet received any treatment was conducted. The results were compared internally and with healthy controls to contribute to the early diagnosis of the disease.METHODS: In this study, the body fluids of 63 patients (30 RIS, 33 MS) and 30 healthy controls were used. From the patient group, samples of cerebrospinal fluid (CSF), serum, and urine; from the healthy group, blood and urine were collected. Metabolomic profiles of the body fluids were generated using Nuclear Magnetic Resonance spectroscopy (NMRS). Multivariate statistics were conducted on the NMRS intensity data using the MetaboAnalyst R package after auto-scaling and log-transformation.RESULTS: In CSF levels of lactate, creatine phosphate, and pyruvate; in serum, levels of hydroxyvalerate, xylitol, and agmatine; in urine threonine, creatine, cystine, 2-aminobutyrate, and ascorbic acid were found significantly higher in the MS group compared to RIS (p ≤ 0.05). In Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA), it was observed that there was not enough differentiation between these two groups. Enrichment Analysis was performed on the CSF results of the RIS group, it was highly consistent with MS disease (ratio=∼1.8).CONCLUSION: Literature reveals various results in this regard; however, the findings here emphasize a new distinction. It's important not to expect a single biomarker to stand out in metabolomic profiling methods; instead, the patient's overall results should be collectively evaluated to conduct a comprehensive analysis. The collective findings of RIS patients being consistent with MS indicate the necessity of widespread adoption and utilization of NMRS technique and metabolomic profiling, especially for CSF, in MS diagnostic criteria. Furthermore, this study provides laboratory evidence suggesting that RIS patients constitute a subtype of MS.PMID:39764909 | DOI:10.1016/j.msard.2024.106250

Aquat Toxicol. 2025 Jan 4;279:107236. doi: 10.1016/j.aquatox.2025.107236. Online ahead of print.ABSTRACTSynthetic progestin dydrogesterone is widely used in gynecology and animal husbandry, leading to high environmental detection rates and concentrations. Dydrogesterone influences sex differentiation, gonad development, and spawning in fish. However, its impact on the liver, a vital organ for hormone production and detoxification, remains largely unknown. In this study, zebrafish embryos were exposed to 2.8, 27.6, and 289.8 ng/L of dydrogesterone until they reached sexual maturity. Metabolomics and Fourier transform infrared spectroscopy (FTIR) were employed to investigate alterations in the zebrafish liver. Long-term exposure to dydrogesterone decreased body weight and length in females but increased them in males. The levels of phospholipids, monoglycerides, lysophospholipids, fatty acids, acylcarnitines, acyltaurines, cholesterol, and bile acids increased in the liver of females but decreased in males due to dydrogesterone, making the metabolic pathways the most affected. FTIR analysis revealed a reduction in lipid and protein absorption coupled with an increase in carbohydrate absorption in the liver of exposed males, whereas exposed females exhibited reductions in both lipid and carbohydrate absorption. These findings suggest that long-term exposure to dydrogesterone enhances basic metabolism and physical growth in male zebrafish. To the best of our knowledge, this is the first report on the effects of progestins on body metabolism. Additionally, we find that gender difference is a notable feature of the effects of dydrogesterone on zebrafish.PMID:39764904 | DOI:10.1016/j.aquatox.2025.107236

Food Chem. 2024 Dec 28;470:142574. doi: 10.1016/j.foodchem.2024.142574. Online ahead of print.ABSTRACTThis study aimed to investigate the effects of fermented milk co-fermented with Lactobacillus (L.) helveticus and commercial starter during storage. Thus, systematic analysis revealed the changes with the determination of physicochemical characteristics, functional properties, and metabolome of fermented milk produced by commercial starter Mild 1.0, L. helveticus H11 (H11), and their combination. Co-fermentation with H11 significantly reduced fermentation time and enhanced pH, titratable acidity, viscosity, water-holding capacity, viable counts of H11, sensory attributes, angiotensin-converting enzyme inhibitory activity, valine-proline-proline and isoleucine-proline-proline levels, and storage stability. Additionally, co-fermentation with H11 enriched seven specific flavor compounds (5-tricosyl-1,3-benzenediol, didodecyl thiobispropanoate, glabrone, tuberoside, isomangiferin, indole-5,6-quinone, and luteone 7-glucoside) and five functional metabolites (indolelactic acid, glycine-histidine, stachyose, riboflavin, and asparagusic acid). These findings established H11 as a valuable adjunct culture for the application of commercial starter to produce functional fermented dairy products.PMID:39764886 | DOI:10.1016/j.foodchem.2024.142574

Poult Sci. 2024 Dec 31;104(2):104754. doi: 10.1016/j.psj.2024.104754. Online ahead of print.ABSTRACTThe activity of intestinal stem cells (ISCs) can be modulated by Lactobacillus, which subsequently affects the mucosal absorptive capacity. However, the underlying mechanisms remain unclear. In this study, a total of 189 Hy-Line Brown chickens (Gallus) were randomly assigned to one of seven experimental groups (n = 27 per group). These groups included a control group, a vehicle group (MRS group), a Lactobacillus salivarius group, a L. salivarius supernatant group, and three succinate treatment groups with various dosages. Each group was further subdivided into three replicates, with 9 chickens per replicate. The results indicate that the administration of Lactobacillus salivarius supernatant to laying hens notably increased the mRNA abundance of the amino acid transporters oligopeptide transporter 1 (PepT1) and sodium-dependent neutral amino acid transporter (B0AT). Metabolomic analyses indicated that the supernatant contains a high concentration of organic acids. Among them, succinate could enhance mRNA abundance of PepT1, B0AT and excitatory amino acid transporters 3 (EAAT3) both in vivo and in vitro. Accordingly, succinate could accelerate intestinal epithelial turnover, as indicated by the increased levels of cyclin-dependent kinase 2 (Cdk2) mRNA and proliferating cell nuclear antigen protein (PCNA), as well as ISC differentiation-related protein leucine-rich repeat containing G protein-coupled receptor 5 (LGR5). Furthermore, succinate treatment was shown to elevate the levels of mitochondrial fusion proteins optic atrophy 1 (OPA1) and translocase of outer mitochondrial membrane 20 (TOMM20), resulting in increased local ATP levels. However, pretreatment with NF-56-EJ40, a succinate receptor antagonist, attenuated the effects of succinate on OPA1, TOMM20, and ATP levels, alone with the reducing LGR5 and PCNA levels. Collectively, succinate, a metabolite of L. salivarius, activates the SUCNR1-mitochondria axis in ISCs, facilitating mitochondrial ATP synthesis, promoting ISC activity, and ultimately enhancing mucosal absorptive capacity.PMID:39764876 | DOI:10.1016/j.psj.2024.104754

Genet Epidemiol. 2025 Jan;49(1):e22607. doi: 10.1002/gepi.22607.ABSTRACTLarge-scale gene-environment interaction (GxE) discovery efforts often involve analytical compromises for the sake of data harmonization and statistical power. Refinement of exposures, covariates, outcomes, and population subsets may be helpful to establish often-elusive replication and evaluate potential clinical utility. Here, we used additional datasets, an expanded set of statistical models, and interrogation of lipoprotein metabolism via nuclear magnetic resonance (NMR)-based lipoprotein subfractions to refine a previously discovered GxE modifying the relationship between physical activity (PA) and HDL-cholesterol (HDL-C). We explored this GxE in the Women's Genome Health Study (WGHS; N = 23,294; the strongest cohort-specific signal in the original meta-analysis), the UK Biobank (UKB; N = 281,380), and the Multi-Ethnic Study of Atherosclerosis (MESA; N = 4587), using self-reported PA (MET-min/wk) and genotypes at rs295849 (nearest gene: LHX1). As originally reported, minor allele carriers of rs295849 in WGHS had a stronger positive association between PA and HDL-C (pint = 0.002). When testing available NMR metabolites to refine the HDL-C outcome, we found a stronger interaction effect on medium-sized HDL particle concentrations (M-HDL-P; pint = 1.0 × 10-4) than HDL-C. Meta-regression revealed a systematically larger interaction effect in cohorts from the original meta-analysis with a greater fraction of women (p = 0.018). In the UKB, GxE effects were stronger in women and using M-HDL-P as the outcome. In MESA, the primary interaction for HDL-C showed nominal significance (pint = 0.013), but without clear sex differences and with a greater magnitude for large HDL-P. Our work provides additional insights into a known gene-PA interaction while illustrating the importance of phenotype and model refinement toward understanding and replicating GxEs.PMID:39764704 | DOI:10.1002/gepi.22607