Integrative Molecular Phenotyping
INTEGRATIVE MOLECULAR
PHENOTYPING
WHEELOCK LABORATORY
DEPARTMENT OF MEDICAL
BIOCHEMISTRY AND BIOPHYSICS
WHEELOCK LABORATORY
DEPARTMENT OF MEDICAL
BIOCHEMISTRY AND BIOPHYSICS
WHEELOCK LABORATORY
DEPARTMENT OF MEDICAL
BIOCHEMISTRY AND BIOPHYSICS
WHEELOCK LABORATORY
DEPARTMENT OF MEDICAL
BIOCHEMISTRY AND BIOPHYSICS
WHEELOCK LABORATORY
DEPARTMENT OF MEDICAL
BIOCHEMISTRY AND BIOPHYSICS
WHEELOCK LABORATORY

Available Positions

2 Positions available

Doctoral (PhD) student position in asthma research: functional metabolomics of lipid mediators in chronic respiratory disease

 

Area of responsibilities/description of the work 
We have an opening for an individual wishing to join a multidisciplinary and translational project aimed to understand mechanisms in obstructive lung disease. Chronic lung disease is one of the largest noncommunicable diseases globally, with 4 million deaths in 2012 alone. Omega 3 fatty acids are found naturally in fish oil and are commonly thought to possess anti-inflammatory properties, with protective effects in inflammatory diseases including asthma. The mechanisms of these effects remain mostly unknown, but are of great interest for their potential therapeutic applications. Specialized pro-resolving mediators (SPMs: protectins, resolvins, and maresins) are generated from omega 3 fatty acids via multiple enzymatic reactions. Several reports have indicated that SPM biosynthesis is impaired in severe asthma, suggesting that chronic inflammation in the lung might result from a resolution defect.

 

This project will use mass spectrometry-based methods to quantify the lipid mediator profile in human lung to determine its ability to synthesize these pro-resolving mediators. Functional studies will be performed on ex vivo lung tissue to evaluate the ability of the omega 3-derived lipid mediators to reduce bronchoconstriction. These studies will be performed in combination with common allergens and bronchoprovacations. Investigations will also be performed in multiple cell types to generate a metabolic profile of both pro-inflammatory and pro-resolving lipid mediators in the human lung. This work will be combined with lipidomics-based profiling to create a lipid map of the human lung. The identified lipid mediator profiles will be used to guide systems medicine-based studies to stratify patients in order to understand the mechanisms driving this heterogeneous disease, with the aim of identifying sub-phenotypes to inform patient diagnosis and treatment strategies.

 

This doctoral position is within the collaborative agreement between the Karolinska Institute (KI) and the National University of Singapore (NUS), and the student will accordingly be admitted to both Universities (http://ki.se/en/collaboration/ki-and-national-university-of-singapore). Students will be jointly taught, supervised, assessed, and will be awarded a PhD degree from both NUS and KI, issued separately. The successful applicant will belong to the Integrative Molecular Phenotyping laboratory led by Dr. Craig Wheelock in the Department of Medical Biochemistry and Biophysics at KI (http://metabolomics.se) and the SLING lipidomics group led by Dr. Markus Wenk at NUS (http://www.lipidprofiles.com). This joint position requires that the student spend at least 12 months over the four year PhD period in residence at NUS.

 

Requirements for entry to doctoral education at KI 
A doctoral position is an available doctoral education project to which a PhD student is to be recruited. After the selection process the process for admission to doctoral education will commence. An admission decision will not be taken until an individual study plan has been approved by the departmental admission board at both Universities, usually within the first six months of the project. For more information regarding doctoral (PhD) education in general, please go to http://www.ki.se/doctoral, and for general and specific entry requirements: http://ki.se/en/education/entry-requirements-eligibility-for-doctoral-education 

 

General entry requirements 
A person meets the general entry requirements for doctoral/third-cycle/PhD education (according to Higher Education Ordinance Chapt 7, section 39) if he/she:

has been awarded advanced/second-cycle/master qualification (i.e. master degree) or
has satisfied the requirements for courses comprising at least 240 credits of which at least 60 credits were awarded in the second-cycle/master level, or
has acquired substantially equivalent knowledge in some other way in Sweden or abroad.


Specific entry requirements Proficiency in English equivalent to the course English B/English 6 at Swedish upper secondary school: Proficiency in the English language can be documented by an internationally recognized test such as TOEFL or IELTS, see web-link below for more information. Applicants who meet the general entry requirements (1 or 2 above) from a university in one of the Nordic countries fulfill the requirements in English. For this particular position, fluency in spoken and written English is a prerequisite as the work environment is international (see below). 

 

Specific entry requirements

Proficiency in English equivalent to the course English B/English 6 at Swedish upper secondary school: Proficiency in the English language can be documented by an internationally recognized test such as TOEFL or IELTS, see web-link below for more information. Applicants who meet the general entry requirements (1 or 2 above) from a university in one of the Nordic countries fulfill the requirements in English. For this particular position, fluency in spoken and written English is a prerequisite as the work environment is international (see below). 

 

Required skills and personal qualities 
Broad biomedical undergraduate training is desired. Practical experience of laboratory work, and in particular experience in analytical chemistry is a useful merit, but there are no pre-determined exclusion criteria. We welcome all applicants that want to have fun and work in a stimulating co-operative international environment to address a new challenge in cutting-edge respiratory research. You should like team-work and international contacts, yet be prepared to assume responsibility to deliver on your own parts in the project and fulfil the requirements of the doctoral training programme. The daily working environment will include cell and tissue culture-based in vivo and in vitro methods for evaluating airway responses as well as instrumental work for lipid quantification.

 

Research environment
Karolinska Institute is one of the world´s leading medical universities. Its mission is to contribute to the improvement of human health through research and education. Karolinska Institute accounts for over 40 per cent of the medical academic research conducted in Sweden, and offers the country´s broadest range of education in medicine and health sciences. Since 1901, the Nobel Assembly at Karolinska Institute has selected the Nobel laureates in Physiology or Medicine. The Department of Medical Biochemistry and Biophysics (MBB) is known for ground-breaking research and responsibility for extensive biomedical education programmes at the under- and postgraduate level. The Nobel Prize in Physiology or Medicine was awarded in 1982 to the former head of the Division of Physiological Chemistry 2 at MBB for key discoveries in eicosanoid research closely related to the current project. The National University of Singapore is consistently ranked as Asia's top university, with a major focus on the biomedical and life sciences. 

 

Application process 
An application must contain the following documents in English:

A personal letter (no longer than 2 A4 pages, font size 11 or larger), explaining why the applicant wants to do a doctoral education, with specification of previous experiences that may contribute to the development of the proposed project, and declaration of how soon you can start.
A curriculum vitae focusing on experiences of relevance to this position
A copy of degree certificates and associated certificates
A copy of degree projects and any previous publications
Interested applicants should contact Craig Wheelock. 
Last application date: 2018-04-30. 

 

Karolinska Institutet is one of the world´s leading medical universities. Its mission is to contribute to the improvement of human health through research and education. Karolinska Institutet accounts for over 40 per cent of the medical academic research conducted in Sweden and offers the country´s broadest range of education in medicine and health sciences. Since 1901 the Nobel Assembly at Karolinska Institutet has selected the Nobel laureates in Physiology or Medicine.

 

Pursuant to the regulations of the Swedish National Archives, applications are kept on file for two years after the appointment has gained legal force. The regulations do not apply to attachments that have been printed or otherwise published.

Karolinska Institutet strives to provide a workplace that has approximately the same number of women and men, is free of discrimination and offers equal opportunity to everyone.

 

For temp agencies and recruiters, and to salespersons: We politely, yet firmly, decline direct contact with temp agencies and recruiters, as well as those selling additional job announcements.
Type of employment    PhD placement
Contract type    Full time
Reference number    2-361/2017
Contact    
Craig Wheelock, +46 (0)8-524 800 00
Union representative    
Anne Edgren, OFR/S, P, O, +46 (0)8-616 16 29
Biborka Bereczky Veress, SACO, +46 (0)70-173 85 75
Henry Wölling, SEKO, +46 (0)8-524 840 80
Last application date    30.Apr.2018 11:59 PM CET

 

Postdoctoral Researcher in Metabolomics of Pulmonary Medicine

 

Area of responsibilities/description of the work:

We have an opening for an individual wishing to join a multidisciplinary team aiming to understand mechanisms in asthma, COPD and other respiratory diseases. The successful candidate will be expected to use and further develop LC-MS-based methods for both non-targeted and targeted metabolomics. The primary focus of the position is to perform metabolomics analyses for the molecular sub-phenotyping of obstructive lung disease in multiple large cohort studies. In particular, we aim to examine the relationship between the onset of asthma and early-life environmental exposure in an exposome approach. This work will be performed in birth cohorts designed to correlate antenatal and postnatal exposure with disease etiology. The acquired metabolomics data will be integrated into an overall “handprint” of ‘omics data including lipidomics, proteomics and transcriptomics in combination with epigenetics to identify distinct molecular signatures of disease sub-phenotypes, with a focus on identifying populations that are sensitive to environmental exposure. Accordingly, the selected candidate will have significant opportunity to interact with other analytical groups and bioinformatics efforts from multiple laboratories internationally. 
The successful applicant will belong to the Integrative Molecular Phenotyping laboratory (http://metabolomics.se) in the Department of Medical Biochemistry and Biophysics. The Department has a strong and internationally recognized research tradition in the physiology and pathobiology of bioactive lipids, including awarding of the Noble Prize for the discovery of eicosanoids. The research environment includes working with state-of-the-art equipment, including multiple mass spectrometers. This project is funded by the Swedish Heart-Lung Foundation. This full-time position is temporary (two years), with potential for renewal for at least 2 more years.

Qualifications

We are looking for highly motivated candidates with a Ph.D. in mass spectrometry or bioanalytical chemistry and experience in small molecule mass spectrometry as well as biological sample preparation techniques. Candidates should have demonstrated experience in LC-MS based metabolomics. We are especially interested in candidates with prior experience working with large clinical studies. In addition, the applicant must have significant and demonstrable experience in dealing with data acquisition, analysis protocols and associated software. Experience with computer programming (e.g., R, Matlab) and multivariate statistics (e.g., SIMCA) would be highly advantageous. Excellent communication skills and an ability to interact socially and scientifically with other post docs and students in the laboratory and with collaborators in various networks are essential. Previous post doc experience and a strong publication record are strong merits.

Start date: 
Funding is available immediately, and the ideal candidate would be able to start as soon as possible. This position is initially available for 24 months, with potential renewal for at least an additional 24 months based upon mutual agreement.

Application process: 
Applications including research experience, CV and at least two references should be submitted through the Varbi recruitment system.

Further Information: 
For additional details of the department and research interests:
http://www.metabolomics.se/ 
http://www.mbb.ki.se 
http://ki.se/en/startpage

Karolinska Institutet is one of the world’s leading medical universities. Its vision is to significantly contribute to the improvement of human health. Karolinska Institutet accounts for the single largest share of all academic medical research conducted in Sweden and offers the country’s broadest range of education in medicine and health sciences. The Nobel Assembly at Karolinska Institutet selects the Nobel laureates in Physiology or Medicine.
Pursuant to the regulations of the Swedish National Archives, applications are kept on file for two years after the appointment has gained legal force. The regulations do not apply to attachments that have been printed or otherwise published.
Karolinska Institutet strives to provide a workplace that has approximately the same number of women and men, is free of discrimination and offers equal opportunity to everyone.

For temp agencies and recruiters, and to salespersons: We politely, yet firmly, decline direct contact with temp agencies and recruiters, as well as those selling additional job announcements.